The myriad spectrum of leptospirosis makes it difficult to confirm
a diagnosis without adequate laboratory facility. Ideally a microbiological
confirmation should be sought for, however, it is not always possible,
as many laboratories do not have the necessary expertise. In such situations
simple clinical, epidemiological and biochemical findings can be of
great help in the diagnosis of infection.
After infection, leptospires are present in the blood until they
are cleared after 4 to 7 days following the production of anti-Leptospira
antibodies, initially mainly of the IgM class. The laboratory diagnosis
of leptospirosis mainly relies on the detection of these anti-Leptospira
antibodies in patients' serum. Several methods are available for
this
purpose:
· The microscopic agglutination test (MAT) is the cornerstone of the
serodiagnosis of leptospirosis, because this assay has a high sensitivity
and allows for the detection of group-specific antibodies.
· Enzyme-Linked ImmunoSorbent Assay (ELISA) or indirect fluorescent antibody
tests (IFAT) have a capacity to detect with a high sensitivity specific IgM antibodies
as a sign
of current or recent leptospirosis.
· PCR proved to be a successful method to detect Leptospira DNA in serum
and even better in urine in the first week of infection. It can also
be used to detect Leptospira DNA in tissues for post-mortem diagnosis,
which are useful when conventional methods like culture fail.
· New Lepto dipstick test is also available
